Description: Rabbit polyclonal antibody to EPN1 Immunogen: Recombinant fusion protein of human EPN1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: EPN1 Alternative Names: Epsin-1; EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1Entrez Gene (Human): 29924Entrez Gene (Mouse) : 13854Entrez Gene (Rat) : 117277SwissProt (Human): Q9Y6I3SwissProt (Mouse) : Q80VP1SwissProt (Rat) : O88339Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EPN1 expression in U87MG (A), mouse lung (B), mouse liver (C), rat brain (D) whole cell lysates.
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Immunohistochemical analysis of EPN1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.