Description: Rabbit polyclonal antibody to ECH1 Immunogen: Recombinant fusion protein of human ECH1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ECH1 Alternative Names: Delta(3 5)-Delta(2 4)-dienoyl-CoA isomerase mitochondrialEntrez Gene (Human): 1891Entrez Gene (Mouse) : 51798Entrez Gene (Rat) : 64526SwissProt (Human): Q13011SwissProt (Mouse) : O35459SwissProt (Rat) : Q62651Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ECH1 expression in MCF7 (A), mouse testis (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of ECH1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.