Description: Rabbit polyclonal antibody to DIS3L Immunogen: Recombinant fusion protein of human DIS3L. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: DIS3L Alternative Names: DIS3L1; KIAA1955; DIS3-like exonuclease 1Entrez Gene (Human): 115752Entrez Gene (Mouse) : 213550Entrez Gene (Rat) : 363077SwissProt (Human): Q8TF46SwissProt (Mouse) : Q8C0S1SwissProt (Rat) : Q5U2P0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DIS3L expression in HeLa (A), mouse testis (B), rat thymus (C) whole cell lysates.
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Immunohistochemical analysis of DIS3L staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.