Description: Rabbit polyclonal antibody to CYR61 Immunogen: Recombinant fusion protein of human CYR61. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: CYR61 Alternative Names: CCN1; GIG1; IGFBP10; Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1Entrez Gene (Human): 3491Entrez Gene (Mouse) : 16007Entrez Gene (Rat) : 83476SwissProt (Human): O00622SwissProt (Mouse) : P18406SwissProt (Rat) : Q9ES72Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CYR61 expression in A375 (A), mouse kidney (B) whole cell lysates.
-
Immunofluorescent analysis of CYR61 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).