Description: Rabbit polyclonal antibody to CLEC-6 Immunogen: Recombinant fusion protein of human CLEC-6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: CLEC4D Alternative Names: CLECSF8; MCL; C-type lectin domain family 4 member D; C-type lectin superfamily member 8; C-type lectin-like receptor 6; CLEC-6Entrez Gene (Human): 338339Entrez Gene (Mouse) : 17474Entrez Gene (Rat) : 362432SwissProt (Human): Q8WXI8SwissProt (Mouse) : Q9Z2H6SwissProt (Rat) : Q69FH1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CLEC-6 expression in HEK293T (A) whole cell lysates.
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  Immunofluorescent analysis of CLEC-6 staining in mouse spleen. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).