Description: Rabbit polyclonal antibody to CaMK2 alpha Immunogen: KLH-conjugated synthetic peptide of human CaMK2 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: CAMK2A Alternative Names: CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alphaEntrez Gene (Human): 815Entrez Gene (Mouse) : 12322Entrez Gene (Rat) : 25400SwissProt (Human): Q9UQM7SwissProt (Mouse) : P11798SwissProt (Rat) : P11275Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CaMK2 alpha expression in HEK293T (A), mouse brain (B), rat lung (C) whole cell lysates.
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Immunofluorescent analysis of CaMK2 alpha staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.