Description: Rabbit polyclonal antibody to RNF52 Immunogen: Recombinant fusion protein of human RNF52. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: BRAP Alternative Names: RNF52; BRCA1-associated protein; BRAP2; Impedes mitogenic signal propagation; IMP; RING finger protein 52; Renal carcinoma antigen NY-REN-63Entrez Gene (Human): 8315Entrez Gene (Mouse) : 72399SwissProt (Human): Q7Z569SwissProt (Mouse) : Q99MP8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RNF52 expression in HepG2 (A), mouse brain (B), rat testis (C) whole cell lysates.
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  Immunohistochemical analysis of RNF52 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.