Description: Rabbit polyclonal antibody to Aph-1 alpha Immunogen: KLH-conjugated synthetic peptide of human Aph-1 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: APH1A Alternative Names: PSF; Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factorEntrez Gene (Human): 51107Entrez Gene (Mouse) : 226548SwissProt (Human): Q96BI3SwissProt (Mouse) : Q8BVF7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Aph-1 alpha expression in U937 (A), HT29 (B), HeLa (C) whole cell lysates.
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  Immunofluorescent analysis of Aph-1 alpha staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.