Description: Rabbit polyclonal antibody to ANO2 Immunogen: Recombinant fusion protein of human ANO2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ANO2 Alternative Names: C12orf3; TMEM16B; Anoctamin-2; Transmembrane protein 16BEntrez Gene (Human): 57101Entrez Gene (Mouse) : 243634SwissProt (Human): Q9NQ90SwissProt (Mouse) : Q8CFW1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ANO2 expression in HeLa (A) whole cell lysates.
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  Immunohistochemical analysis of ANO2 staining in mouse retina formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.