Description: Rabbit polyclonal antibody to ANKLE2 Immunogen: Recombinant fusion protein of human ANKLE2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ANKLE2 Alternative Names: KIAA0692; LEM4; Ankyrin repeat and LEM domain-containing protein 2; LEM domain-containing protein 4Entrez Gene (Human): 23141Entrez Gene (Mouse) : 71782Entrez Gene (Rat) : 360829SwissProt (Human): Q86XL3SwissProt (Mouse) : Q6P1H6SwissProt (Rat) : Q7TP65Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ANKLE2 expression in Jurkat (A) whole cell lysates.
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Immunohistochemical analysis of ANKLE2 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.