Description: Rabbit polyclonal antibody to ALPL Immunogen: KLH-conjugated synthetic peptide of human ALPL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ALPL Alternative Names: Alkaline phosphatase tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozymeEntrez Gene (Human): 249Entrez Gene (Mouse) : 11647Entrez Gene (Rat) : 25586SwissProt (Human): P05186SwissProt (Mouse) : P09242SwissProt (Rat) : P08289Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ALPL expression in U87MG (A) whole cell lysates.
• 
  Immunohistochemical analysis of ALPL staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.