Description: Rabbit polyclonal antibody to ALG1 Immunogen: Recombinant fusion protein of human ALG1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: ALG1 Alternative Names: HMAT1; HMT1; Chitobiosyldiphosphodolichol beta-mannosyltransferase; Asparagine-linked glycosylation protein 1 homolog; Beta-1 4-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; Mannosyltransferase-1; MT-1; hMat-1Entrez Gene (Human): 56052Entrez Gene (Mouse) : 208211SwissProt (Human): Q9BT22SwissProt (Mouse) : Q921Q3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ALG1 expression in HepG2 (A), mouse kidney (B), rat liver (C) whole cell lysates.
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  Immunofluorescent analysis of ALG1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.