Description: Rabbit polyclonal antibody to AFG3L2 Immunogen: Recombinant fusion protein of human AFG3L2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: AFG3L2 Alternative Names: AFG3-like protein 2; Paraplegin-like proteinEntrez Gene (Human): 10939Entrez Gene (Mouse) : 69597SwissProt (Human): Q9Y4W6SwissProt (Mouse) : Q8JZQ2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AFG3L2 expression in HeLa (A), mouse brain (B), mouse heart (C), mouse kidney (D) whole cell lysates.
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Immunohistochemical analysis of AFG3L2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.