Description: Rabbit polyclonal antibody to ADI1 Immunogen: Recombinant fusion protein of human ADI1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ADI1 Alternative Names: MTCBP1; 1 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD; Fe-ARD; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; MTCBP-1; Submergence-induced protein-like factor; Sip-LEntrez Gene (Human): 55256Entrez Gene (Mouse) : 104923Entrez Gene (Rat) : 298934SwissProt (Human): Q9BV57SwissProt (Mouse) : Q99JT9SwissProt (Rat) : Q562C9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ADI1 expression in HT29 (A), HEK293T (B) whole cell lysates.
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  Immunohistochemical analysis of ADI1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.