Description: Rabbit polyclonal antibody to ACSL1 Immunogen: Recombinant fusion protein of human ACSL1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: ACSL1 Alternative Names: FACL1; FACL2; LACS; LACS1; LACS2; Long-chain-fatty-acid--CoA ligase 1; Acyl-CoA synthetase 1; ACS1; Long-chain acyl-CoA synthetase 1; LACS 1; Long-chain acyl-CoA synthetase 2; LACS 2; Long-chain fatty acid-CoA ligase 2; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2Entrez Gene (Human): 2180Entrez Gene (Mouse) : 14081Entrez Gene (Rat) : 25288SwissProt (Human): P33121SwissProt (Mouse) : P41216SwissProt (Rat) : P18163Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACSL1 expression in HepG2 (A), mouse liver (B), rat heart (C) whole cell lysates.
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Immunofluorescent analysis of ACSL1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).