Description: Rabbit polyclonal antibody to ACF Immunogen: Recombinant fusion protein of human ACF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: A1CF Alternative Names: ACF; ASP; APOBEC1 complementation factor; APOBEC1-stimulating proteinEntrez Gene (Human): 29974Entrez Gene (Mouse) : 69865Entrez Gene (Rat) : 170912SwissProt (Human): Q9NQ94SwissProt (Mouse) : Q5YD48SwissProt (Rat) : Q923K9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ACF expression in HT29 (A), HeLa (B), mouse liver (C) whole cell lysates.
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  Immunohistochemical analysis of ACF staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.