Description: Rabbit polyclonal antibody to EPO Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPO. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: EPO Alternative Names: Erythropoietin; EpoetinEntrez Gene (Human): 2056Entrez Gene (Mouse) : 13856Entrez Gene (Rat) : 24335SwissProt (Human): P01588SwissProt (Mouse) : P07321SwissProt (Rat) : P29676Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EPO expression in SW620 (A), mouse liver (B), rat kidney (C) whole cell lysates.
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  Immunohistochemical analysis of EPO staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.