Anti-CD284 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to CD284 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD284. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: TLR4 Alternative Names: Toll-like receptor 4; hToll; CD284Entrez Gene (Human): 7099Entrez Gene (Mouse) : 21898Entrez Gene (Rat) : 29260SwissProt (Human): O00206SwissProt (Mouse) : Q9QUK6SwissProt (Rat) : Q9QX05Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD284 expression in Jurkat (A), mouse skeletal muscle (B), rat lung (C) whole cell lysates. -
Immunofluorescent analysis of CD284 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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