Description: Rabbit polyclonal antibody to RagD Immunogen: Recombinant full length protein of human RagD Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RRAGD Alternative Names: Ras-related GTP-binding protein D; Rag D; RagDEntrez Gene (Human): 58528Entrez Gene (Mouse) : 52187SwissProt (Human): Q9NQL2SwissProt (Mouse) : Q7TT45Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RagD expression in SHSY5Y (A), mouse heart (B) whole cell lysates.
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Immunohistochemical analysis of RagD staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.