Description: Rabbit polyclonal antibody to TRIP12 Immunogen: Recombinant full length protein of human TRIP12 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TRIP12 Alternative Names: KIAA0045; ULF; E3 ubiquitin-protein ligase TRIP12; E3 ubiquitin-protein ligase for Arf; ULF; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12Entrez Gene (Human): 9320Entrez Gene (Mouse) : 14897Entrez Gene (Rat) : 316575SwissProt (Human): Q14669SwissProt (Mouse) : G5E870SwissProt (Rat) : F1LP64Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TRIP12 expression in Hela (A), Jurkat (B) whole cell lysates.
-
Immunohistochemical analysis of TRIP12 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.