Description: Rabbit polyclonal antibody to RPE65 Immunogen: Recombinant full length protein of human RPE65 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: RPE65 Alternative Names: Retinoid isomerohydrolase; All-trans-retinyl-palmitate hydrolase; Retinal pigment epithelium-specific 65 kDa protein; Retinol isomeraseEntrez Gene (Human): 6121Entrez Gene (Mouse) : 19892Entrez Gene (Rat) : 89826SwissProt (Human): Q16518SwissProt (Mouse) : Q91ZQ5SwissProt (Rat) : O70276Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RPE65 expression in Hela (A), mouse heart (B), rat brain (C) whole cell lysates.
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  Immunofluorescent analysis of RPE65 staining in rat eye. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).