Description: Rabbit polyclonal antibody to NOXA Immunogen: KLH-conjugated synthetic peptide of human NOXA Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: PMAIP1 Alternative Names: NOXA; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; Immediate-early-response protein APR; Protein NoxaEntrez Gene (Human): 5366Entrez Gene (Mouse) : 58801Entrez Gene (Rat) : 492821SwissProt (Human): Q13794SwissProt (Mouse) : Q9JM54SwissProt (Rat) : Q5U777Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NOXA expression in Jurkat (A), Hela (B), SKOV3 (C) whole cell lysates.
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Immunofluorescent analysis of NOXA staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).