Description: Rabbit polyclonal antibody to HFM1 Immunogen: Recombinant full length protein of human HFM1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HFM1 Alternative Names: SEC3D1; Probable ATP-dependent DNA helicase HFM1; SEC63 domain-containing protein 1Entrez Gene (Human): 164045SwissProt (Human): A2PYH4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HFM1 expression in K562 (A) whole cell lysates.
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Immunohistochemical analysis of HFM1 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.