Description: Rabbit polyclonal antibody to Cytochrome P450 46A1 Immunogen: Recombinant full length protein of human Cytochrome P450 46A1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: CYP46A1 Alternative Names: CYP46; Cholesterol 24-hydroxylase; CH24H; Cytochrome P450 46A1Entrez Gene (Human): 10858Entrez Gene (Mouse) : 13116SwissProt (Human): Q9Y6A2SwissProt (Mouse) : Q9WVK8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Cytochrome P450 46A1 expression in MCF7 (A), mouse brain (B), rat liver (C) whole cell lysates.
-
Immunohistochemical analysis of Cytochrome P450 46A1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.