Description: Rabbit polyclonal antibody to SMEK1 Immunogen: Recombinant full length protein of human SMEK1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: SMEK1 Alternative Names: KIAA2010; PP4R3A; PPP4R3A; Serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1Entrez Gene (Human): 55671Entrez Gene (Mouse) : 68734SwissProt (Human): Q6IN85SwissProt (Mouse) : Q6P2K6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMEK1 expression in Jurkat (A), MCF7 (B), mouse spleen (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of SMEK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.