Description: Rabbit polyclonal antibody to Ferrochelatase Immunogen: Recombinant full length protein of human Ferrochelatase Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: FECH Alternative Names: Ferrochelatase mitochondrial; Heme synthase; Protoheme ferro-lyaseEntrez Gene (Human): 2235SwissProt (Human): P22830SwissProt (Mouse) : P22315Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ferrochelatase expression in HT29 (A), mouse brain (B) whole cell lysates.
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Immunohistochemical analysis of Ferrochelatase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.