Description: Rabbit polyclonal antibody to RNF166 Immunogen: KLH-conjugated synthetic peptide of human RNF166 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RNF166 Alternative Names: RING finger protein 166Entrez Gene (Human): 115992Entrez Gene (Mouse) : 68718Entrez Gene (Rat) : 365022SwissProt (Human): Q96A37SwissProt (Mouse) : Q3U9F6SwissProt (Rat) : Q6J1I7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RNF166 expression in SHSY5Y (A), K562 (B), MCF7 (C) whole cell lysates.
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  Immunohistochemical analysis of RNF166 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.