Description: Rabbit polyclonal antibody to ARFGAP3 Immunogen: Recombinant full length protein of human ARFGAP3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ARFGAP3 Alternative Names: ARFGAP1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3Entrez Gene (Human): 26286Entrez Gene (Mouse) : 66251SwissProt (Human): Q9NP61SwissProt (Mouse) : Q9D8S3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ARFGAP3 expression in HepG2 (A), mouse pancreas (B) whole cell lysates.
-
Immunohistochemical analysis of ARFGAP3 staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.