Description: Rabbit polyclonal antibody to KDM6A Immunogen: Recombinant full length protein of human KDM6A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: KDM6A Alternative Names: UTX; Lysine-specific demethylase 6A; Histone demethylase UTX; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat proteinEntrez Gene (Human): 7403Entrez Gene (Mouse) : 22289SwissProt (Human): O15550SwissProt (Mouse) : O70546Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of KDM6A expression in SHSY5Y (A), NIH3T3 (B), mouse spleen (C), rat liver (D) whole cell lysates.
-
Immunohistochemical analysis of KDM6A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.