Description: Rabbit polyclonal antibody to TLF Immunogen: Recombinant full length protein of human TLF Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: TBPL1 Alternative Names: TLF; TLP; TLP21; TRF2; TRP; TATA box-binding protein-like protein 1; TBP-like protein 1; 21 kDa TBP-like protein; Second TBP of unique DNA protein; STUD; TATA box-binding protein-related factor 2; TBP-related factor 2; TBP-like factor; TBP-related proteinEntrez Gene (Human): 9519Entrez Gene (Mouse) : 237336SwissProt (Human): P62380SwissProt (Mouse) : P62340Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TLF expression in HEK293T (A) whole cell lysates.
-
Immunofluorescent analysis of TLF staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.