Description: Rabbit polyclonal antibody to SMAD9 Immunogen: Recombinant full length protein of human SMAD9 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: SMAD9 Alternative Names: MADH6; MADH9; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9Entrez Gene (Human): 4093Entrez Gene (Mouse) : 55994Entrez Gene (Rat) : 85435SwissProt (Human): O15198SwissProt (Mouse) : Q9JIW5SwissProt (Rat) : O54835Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SMAD9 expression in HepG2 (A), A549 (B) whole cell lysates.
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Immunohistochemical analysis of SMAD9 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of SMAD9 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.