Description: Rabbit polyclonal antibody to SPAG16 Immunogen: Recombinant full length protein of human SPAG16 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SPAG16 Alternative Names: PF20; Sperm-associated antigen 16 protein; Pf20 protein homologEntrez Gene (Human): 79582Entrez Gene (Mouse) : 66722SwissProt (Human): Q8N0X2SwissProt (Mouse) : Q8K450Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SPAG16 expression in mouse testis (A) whole cell lysates.
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  Immunohistochemical analysis of SPAG16 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.