Description: Rabbit polyclonal antibody to CARD12 Immunogen: Recombinant full length protein of human CARD12 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: NLRC4 Alternative Names: CARD12; CLAN; CLAN1; IPAF; NLR family CARD domain-containing protein 4; CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; IpafEntrez Gene (Human): 58484Entrez Gene (Mouse) : 268973Entrez Gene (Rat) : 298784SwissProt (Human): Q9NPP4SwissProt (Mouse) : Q3UP24SwissProt (Rat) : F1M649Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates.
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Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.