Description: Rabbit polyclonal antibody to CARD10 Immunogen: Recombinant full length protein of human CARD10 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: CARD10 Alternative Names: CARMA3; Caspase recruitment domain-containing protein 10; CARD-containing MAGUK protein 3; Carma 3Entrez Gene (Human): 29775Entrez Gene (Mouse) : 105844SwissProt (Human): Q9BWT7SwissProt (Mouse) : P58660Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CARD10 expression in HT29 (A), Jurkat (B), mouse heart (C) whole cell lysates.
-
Immunohistochemical analysis of CARD10 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.