Description: Rabbit polyclonal antibody to AMPK alpha 2 Immunogen: Recombinant full length protein of human AMPK alpha 2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PRKAA2 Alternative Names: AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinaseEntrez Gene (Human): 5563Entrez Gene (Mouse) : 108079Entrez Gene (Rat) : 78975SwissProt (Human): P54646SwissProt (Mouse) : Q8BRK8SwissProt (Rat) : Q09137Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AMPK alpha 2 expression in SW620 (A), mouse brain (B), rat heart (C) whole cell lysates.
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Immunofluorescent analysis of AMPK alpha 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.