Description: Rabbit polyclonal antibody to EAF2 Immunogen: Recombinant full length protein of human EAF2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: EAF2 Alternative Names: TRAITS; ELL-associated factor 2; Testosterone-regulated apoptosis inducer and tumor suppressor proteinEntrez Gene (Human): 55840Entrez Gene (Mouse) : 106389Entrez Gene (Rat) : 266787SwissProt (Human): Q96CJ1SwissProt (Mouse) : Q91ZD6SwissProt (Rat) : Q811X5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EAF2 expression in HepG2 (A), A431 (B), mouse kidney (C), rat kidney (D) whole cell lysates.
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  Immunofluorescent analysis of EAF2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.