Description: Rabbit polyclonal antibody to VPS4A Immunogen: Recombinant full length protein of human VPS4A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: VPS4A Alternative Names: VPS4; Vacuolar protein sorting-associated protein 4A; Protein SKD2; VPS4-1; hVPS4Entrez Gene (Human): 27183Entrez Gene (Mouse) : 116733Entrez Gene (Rat) : 246772SwissProt (Human): Q9UN37SwissProt (Mouse) : Q8VEJ9SwissProt (Rat) : Q793F9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of VPS4A expression in MCF7 (A), Hela (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of VPS4A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of VPS4A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.