Description: Rabbit polyclonal antibody to TRMT2A Immunogen: KLH-conjugated synthetic peptide of human TRMT2A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TRMT2A Alternative Names: HTF9C; tRNA (uracil-5-)-methyltransferase homolog A; HpaII tiny fragments locus 9c proteinEntrez Gene (Human): 27037SwissProt (Human): Q8IZ69Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of TRMT2A expression in HL60 (A), Raji (B) whole cell lysates.
-
Immunohistochemical analysis of TRMT2A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.