Description: Rabbit polyclonal antibody to HAND2 Immunogen: Recombinant full length protein of human HAND2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: HAND2 Alternative Names: BHLHA26; DHAND; Heart- and neural crest derivatives-expressed protein 2; Class A basic helix-loop-helix protein 26; bHLHa26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHANDEntrez Gene (Human): 9464Entrez Gene (Mouse) : 15111Entrez Gene (Rat) : 64637SwissProt (Human): P61296SwissProt (Mouse) : Q61039SwissProt (Rat) : P61295Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HAND2 expression in mouse liver (A), mouse heart (B) whole cell lysates.
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Immunofluorescent analysis of HAND2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.