Description: Rabbit polyclonal antibody to HPGD Immunogen: Recombinant full length protein of human HPGD Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HPGD Alternative Names: PGDH1; 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; 15-PGDH; Prostaglandin dehydrogenase 1Entrez Gene (Human): 3248Entrez Gene (Mouse) : 15446Entrez Gene (Rat) : 79242SwissProt (Human): P15428SwissProt (Mouse) : Q8VCC1SwissProt (Rat) : O08699Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HPGD expression in Jurkat (A), mouse liver (B), mouse lung (C) whole cell lysates.
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Immunohistochemical analysis of HPGD staining in rat intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.