Description: Rabbit polyclonal antibody to CD249 Immunogen: Recombinant full length protein of human CD249 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ENPEP Alternative Names: Glutamyl aminopeptidase; EAP; Aminopeptidase A; AP-A; Differentiation antigen gp160; CD249Entrez Gene (Human): 2028Entrez Gene (Mouse) : 13809Entrez Gene (Rat) : 64017SwissProt (Human): Q07075SwissProt (Mouse) : P16406SwissProt (Rat) : P50123Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD249 expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of CD249 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.