Description: Rabbit polyclonal antibody to ASGR1 Immunogen: Recombinant full length protein of human ASGR1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ASGR1 Alternative Names: CLEC4H1; Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1Entrez Gene (Human): 432Entrez Gene (Mouse) : 11889Entrez Gene (Rat) : 24210SwissProt (Human): P07306SwissProt (Mouse) : P34927SwissProt (Rat) : P02706Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ASGR1 expression in HepG2 (A), mouse liver (B), rat lung (C) whole cell lysates.
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Immunohistochemical analysis of ASGR1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.