Description: Rabbit polyclonal antibody to 15-LO Immunogen: Recombinant full length protein of human 15-LO Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ALOX15 Alternative Names: LOG15; Arachidonate 15-lipoxygenase; 15-LOX; 15-LOX-1; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type; 12-LOX; Arachidonate omega-6 lipoxygenaseEntrez Gene (Human): 246Entrez Gene (Mouse) : 11687Entrez Gene (Rat) : 81639SwissProt (Human): P16050SwissProt (Mouse) : P39654SwissProt (Rat) : Q02759Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of 15-LO expression in COS7 (A), mouse heart (B) whole cell lysates.
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  Immunohistochemical analysis of 15-LO staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.