Description: Rabbit polyclonal antibody to BCL9 Immunogen: Recombinant full length protein of human BCL9 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: BCL9 Alternative Names: B-cell CLL/lymphoma 9 protein; B-cell lymphoma 9 protein; Bcl-9; Protein legless homologEntrez Gene (Human): 607Entrez Gene (Mouse) : 77578SwissProt (Human): O00512SwissProt (Mouse) : Q9D219Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BCL9 expression in MCF7 (A), mouse pancreas (B) whole cell lysates.
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Immunohistochemical analysis of BCL9 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.