Description: Rabbit polyclonal antibody to Flotillin 2 Immunogen: Recombinant full length protein of human Flotillin 2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: FLOT2 Alternative Names: ESA1; M17S1; Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1Entrez Gene (Human): 2319Entrez Gene (Mouse) : 14252Entrez Gene (Rat) : 83764SwissProt (Human): Q14254SwissProt (Mouse) : Q60634SwissProt (Rat) : Q9Z2S9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Flotillin 2 expression in A549 (A), SKOV3 (B), mouse heart (C), rat brain (D) whole cell lysates.
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Immunofluorescent analysis of Flotillin 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.