Description: Rabbit polyclonal antibody to PRPF4 Immunogen: Recombinant full length protein of human PRPF4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PRPF4 Alternative Names: PRP4; U4/U6 small nuclear ribonucleoprotein Prp4; PRP4 homolog; hPrp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4Entrez Gene (Human): 9128Entrez Gene (Mouse) : 70052SwissProt (Human): O43172SwissProt (Mouse) : Q9DAW6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of PRPF4 expression in HT29 (A), MCF7 (B), mouse testis (C), rat spleen (D) whole cell lysates.
-
Immunohistochemical analysis of PRPF4 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.