Description: Rabbit polyclonal antibody to PIWIL2 Immunogen: Recombinant full length protein of human PIWIL2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PIWIL2 Alternative Names: HILI; Piwi-like protein 2; Cancer/testis antigen 80; CT80Entrez Gene (Human): 55124Entrez Gene (Mouse) : 57746SwissProt (Human): Q8TC59SwissProt (Mouse) : Q8CDG1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PIWIL2 expression in mouse skeletal muscle (A), rat skeletal muscle (B) whole cell lysates.
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Immunohistochemical analysis of PIWIL2 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.