Description: Rabbit polyclonal antibody to eIF2C2 Immunogen: KLH-conjugated synthetic peptide of human eIF2C2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: AGO2 Alternative Names: EIF2C2; Protein argonaute-2; Argonaute2; hAgo2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; PAZ Piwi domain protein; PPD; Protein slicerEntrez Gene (Human): 27161Entrez Gene (Mouse) : 239528Entrez Gene (Rat) : 59117SwissProt (Human): Q9UKV8SwissProt (Mouse) : Q8CJG0SwissProt (Rat) : Q9QZ81Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of eIF2C2 expression in Hela (A), K562 (B), NIH3T3 (C) whole cell lysates.
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Immunohistochemical analysis of eIF2C2 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.