Description: Rabbit polyclonal antibody to HuB Immunogen: Recombinant full length protein of human HuB Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ELAVL2 Alternative Names: HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1Entrez Gene (Human): 1993Entrez Gene (Mouse) : 15569SwissProt (Human): Q12926SwissProt (Mouse) : Q60899SwissProt (Rat) : Q8CH84Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of HuB expression in mouse cerebellun (A) whole cell lysates.
-
Immunohistochemical analysis of HuB staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.