Description: Rabbit polyclonal antibody to TRIM69 Immunogen: Recombinant full length protein of human TRIM69 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: TRIM69 Alternative Names: RNF36; E3 ubiquitin-protein ligase TRIM69; RFP-like domain-containing protein trimless; RING finger protein 36; Tripartite motif-containing protein 69Entrez Gene (Human): 140691Entrez Gene (Mouse) : 70928Entrez Gene (Rat) : 311373SwissProt (Human): Q86WT6SwissProt (Mouse) : Q80X56SwissProt (Rat) : Q5BK82Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRIM69 expression in mouse liver (A) whole cell lysates.
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  Immunohistochemical analysis of TRIM69 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of TRIM69 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.